- Step 1: Open search page Open the Basic Local Alignment Search Tool page from the National Center for Biotechnology Information website by clicking on BLAST in the Popular Resources menu.
- Step 2: Choose program Choose a BLAST program to run from the Basic BLAST menu; read the short descriptions of each program to figure out which one you should run.
- Step 3: Select database On the program page, select the BLAST database you want to use from the Database pull-down menu under Choose Search Set.
- Step 4: Enter sequence Copy and paste the Accession number, GI number, or FASTA formatted sequence into the large text box under Enter Query Sequence.
- TIP: Choose search type Many Accession and FASTA sequences can be located using NCBI’s Entrez Gene site at "ncbi.nlm.nih.gov/sites/entrez?db=gene":http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene. GI numbers can be found using the Sequence Revision History tool at "ncbi.nlm.nih.gov/sviewer/girevhist.cgi?":http://www.ncbi.nlm.nih.gov/sviewer/girevhist.cgi?.
- Step 5: Submit Click on the BLAST button to submit your search; the page will refresh when the results are ready.
- FACT: The first isolation of DNA was performed by Swiss biologist Friedrich Miescher in 1869.
You Will Need