Publish date:
Updated on

How To Prepare DNA For Analysis By Gel Electrophoresis

After DNA has been crystallized, it must be resuspended in order to fit in the wells of a gel for electrophoresis.

Instructions

  • Step 1: Evaporate the ethanol by placing the second tube under a bright light, or place it in a vacuum bell for 15 to 30 minutes.
  • Step 2: Add 100 microliters of TE to the tube with a micropipettor. Flick the tube vigorously to mix. Let the DNA sit for three to four days to resuspend.
  • FACT: In April, 1953, James Watson and Francis Crick first presented the double helix structure of DNA.
  • TIP: Break off the glass hook in the tube if the DNA will not stick to the side.
  • Step 3: Pull the DNA out of the ethanol and transfer it to the empty tube. Stick the DNA to the side of the tube.
  • Step 4: Pick up the DNA with the glass pipette hook and transfer it to the ethanol tube.
  • Step 5: Use the hook to wiggle the DNA around in the tube for one minute.
  • Step 6: Put 1 milliliter of the 95 percent ethanol into the first tube.

Popular Categories